Matchday 6 in Group H sees a youthful Arsenal side travel to the Karaiskakis Stadium to face Greek champions Olympiacos, while Standard Liege host the group's basement boys AZ Alkmaar.
The Gunners have already secured top spot in the group and having defeated Standard Liege 2-0 on Matchday 5, will head into the last 16 draw safe in the knowledge they will avoid the likes of Real Madrid.
With an injury-ravaged squad and strikers Robin Van Persie, Nicklas Bendtner and Eduardo all on the sidelines, boss Arsene Wenger will turn to his youngsters again ahead of a tough trip to Greece.
Olympiakos shot themselves back into contention after a solid 0-0 draw away to AZ Alkmaar last time out which saw them climb up to second in the group on seven points, three ahead of third placed Standard Liege.
Now Zico's men will be confident of securing the three points they need against a young Arsenal side to seal qualification into the last 16 for the second time in three seasons.
Elsewhere, Standard Liege will be hoping for a Gunners win in Athens, which would mean should they beat Dutch champions AZ Alkmaar in Liege, it would be they who grab the second qualification spot.